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Date Name Thumbnail Size Description Versions
14:58, 19 September 2015 BBa K1804001 PlasmidMap.png (file) 49 KB Plasmid map of plac-gfp in pSB1C3 vector (BBa_K1804001). 1
14:47, 19 September 2015 BBa K1804001 FluorescenceIntensities.png (file) 19 KB GFP is expressed in BL21 transformed with pAC-placgfp and pSB1C3. Data is represented in Mean +/- Standard Deviation of GFP fluorescence intensity (A.U.) for the positive control of BL21 pAC-plac-gfp, BL21 pSB1C3-plac-gfp and the negative control BL21. Th 1
14:43, 19 September 2015 BBa K1804001 Descriptives.png (file) 18 KB Descriptive statistics for the fluorescence intensities of the bacteria cells used in the experimental duplicates (n=15). Descriptive statistics for the negative control were not included as the fluorescence intensities of the negative control were consta 1
14:37, 19 September 2015 BBa K1804001 Fluorescence.png (file) 885 KB Representative images of bacterial cultures were taken using a confocal laser scanning microscope at 488 nm, in duplicates A. BL21 pAC-plac-gfp, positive control. B. BL21 pSB1C3-plac-gfp. C. BL21, negative control. 1
14:12, 19 September 2015 BBa K1804001 Figure2 PCR.png (file) 74 KB PCR of correct clone of pSB1C3-plac-gfp using BioBricks Prefix and BioBricks Suffix primers. Marker used is 100 bp ladder. Lanes 1 to 4 are PCRs of pSB1C3-plac-gfp, PCR-purified Junk-Prefix-plac-gfp-Suffix-Junk (positive control), pAC-plac-gfp (negative c 1
14:06, 19 September 2015 BBa K1804001 Figure1.png (file) 151 KB Plasmid maps of pAC plasmids with (A) EsaR-EsaI (original plasmid from Addgene #47660) or (B) plac-gfp 1