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Date Name Thumbnail Size Description Versions
20:36, 26 October 2014 I13522(2).png (file) 15 KB Figure 1 – BBa_I13522 constitutively expresses GFP. Mean green fluorescence (Excitation 485nm/Emission 520nm) of BBa_I13522 transformed E.Coli (circles) and untransformed E. coli (triangles), immobilised on Poly-Lysine. 50μl Chloramphenicol liquid LB, 1
20:33, 26 October 2014 I13522.png (file) 14 KB Figure 1 – BBa_I13522 constitutively expresses GFP. Mean green fluorescence (Excitation 485nm/Emission 520nm) of BBa_I13522 transformed E.Coli (circles) and untransformed E. coli (triangles), immobilised on Poly-Lysine. 50μl Chloramphenicol liquid LB, 1
19:58, 26 October 2014 FIG3-T9002(3).png (file) 14 KB   1
19:57, 26 October 2014 FIG3-T9002(2).png (file) 13 KB   1
15:06, 26 October 2014 FIG5-T9002.png (file) 38 KB T9002 and K1090000 characterisation Surface-immobilisation of sender-receiver QS tranformants results in improved GFP expression. Poly-L-lysine wells (triangles) had greater rate of response compared to cells free in suspension (circles). This was perfor 1
15:05, 26 October 2014 FIG4-T9002.png (file) 40 KB K1090000 and T9002 characterisation K1090000 Sender E. coli induction increased GFP expression in T9002 Receivers. Growth started in cell number ratios of aliquots totalling OD600 0.02 densities, suspended in liquid LB. Untransformed XL1-Blue:Receiver 1: 1
15:04, 26 October 2014 FIG3-T9002(seer).png (file) 50 KB K1090000 and T9002 characterisation Greatest absolute fluorescence by T9002 transformants is observed in a Sender (S) 1:100 Receiver (R) cell number initial ratio. T9002 Receiver-produced GFP was compared with that produced when paired with an untransfor 1
15:01, 26 October 2014 FIG2-T9002.png (file) 38 KB K1090000 and T9002 characterisation Production of GFP by T9002 Receiver, driven by either by filtered AHL-containing culture medium derived from K1090000 cultures, or by actively growing K1090000 sender transformants. AHL derived from culture medium is s 1
15:01, 26 October 2014 FIG1-T9002.png (file) 42 KB T9002 characterisation Figure 1 K1090000 E. coli constitutively expresses RFP. Red (Excitation 544nm/Emission 612nm) fluorescence of K1090000 E. coli (triangles), RFP positive control pSB1C3 (Circles), untransformed XL1-Blue E. coli (dashes). 1