File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 21:48, 18 September 2015 | TU Eindhoven BRET.jpg (file) |  |
50 KB | Bioluminescence Resonance Energy Transfer (BRET) between NanoLuc and mNeonGreen. | 1 |
| 21:45, 18 September 2015 | TU Eindhoven BRET Diagram.jpg (file) |  |
73 KB | Simplified energy-level diagram of RET. Normally, an excited electron falls back to its ground state under the emission of light (a radiative transition). In the case of Resonance Electron Transfer, an excited electron in the donor falls back to its groun | 1 |
| 19:34, 18 September 2015 | TU Eindhoven Bioluminescence Results OmpX-NanoLuc OmpX-mNeonGreen.jpg (file) |  |
86 KB | Bioluminescence results of our complete construct, containing both NanoLuc and mNeonGreen. From this it can be concluded that our construct worked as expected (BRET is taking place). This has been tested with a bioluminescence assay. Furimazine was added. | 1 |
| 19:22, 18 September 2015 | TU Eindhoven Bioluminescence Results OmpX-NanoLuc.jpg (file) |  |
62 KB | Fluorescence results of our OmpX-NanoLuc construct. From this it can be concluded that NanoLuc works as expected. This has been tested through a bioluminescence assay and gave a nice and smooth spectrum as result. An important requirement for this assay i | 1 |
