File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 15:38, 8 October 2022 | N9 cell assay.png (file) |  |
101 KB | Cell assay with THP-1 cells | 1 |
| 15:23, 8 October 2022 | All nbs cell assay.png (file) |  |
192 KB | Cell assay with THP-1 cells | 1 |
| 15:20, 8 October 2022 | N3 cell assay.png (file) |  |
103 KB | Cell assay with THP-1 cells | 1 |
| 15:16, 8 October 2022 | N2 cell assay.png (file) |  |
94 KB | Cell assay with THP-1 cells | 1 |
| 15:11, 8 October 2022 | N1 cell assay.png (file) |  |
102 KB | Cell assay using tHP-1 cells | 1 |
| 13:16, 8 October 2022 | Western and ELISA MC1061.png (file) |  |
2.31 MB | This figure shows a western blot together with the corresponding ELISA, detecting the TNFa-binding capabilities of secreted nanobodies. | 1 |
| 20:10, 7 October 2022 | ELISA Nissle NO.jpeg (file) |  |
55 KB | The ELISA shows that secreted nanobodies, induce with DETA/NO (nitric oxide source), successfully bind TNFalpha. | 1 |
| 14:55, 7 October 2022 | Western & ELISA Nissle.png (file) |  |
1.63 MB | This graph shows a western blot, used to detect secretion of functional nanobodies and the ELISA, showing the binding capabilities of the secreted nanobodies. Secretion was induced by arabinose in the probiotic E. coli Nissle strain. | 1 |
| 14:44, 6 October 2022 | NO induction Nissle.png (file) |  |
760 KB | This Western blot shows the anti-TNFa nanobody expression upon nitric oxide induction in the probiotic strain E. coli Nissle 1917. | 1 |
| 11:14, 6 October 2022 | Secretion MC1061 and Nissle.png (file) |  |
1.07 MB | Graph shows 2 Western blots, proofing the ability of E. coli MC1061 and E. coli Nissle 1917 to successfully secrete monovalent and bivalent nanobodies. | 1 |
| 09:57, 6 October 2022 | ELISA MC1061.jpeg (file) |  |
258 KB | ELISA comparing the binding capabilities of monovalent and bivalent anti-TNF nanobodies, either obtained via purification or directly secreted by E. coli MC1061. | 1 |
| 09:49, 6 October 2022 | Ara ind Nb sec MC1061.png (file) |  |
720 KB | This Western blot shows the secretion of monovalent and bivalent anti-TNF nanobodies in E. coli MC1061. | 1 |
| 16:44, 5 October 2022 | ELISA pur Nbs.jpeg (file) |  |
64 KB | ELISA with purified nanobodies | 1 |
| 16:18, 5 October 2022 | Native PAGE 1.png (file) |  |
1.93 MB | Native PAGE gel of purified nanobodies (first purification attempt) | 1 |
| 16:16, 5 October 2022 | Native PAGE 2.png (file) |  |
2.03 MB | Native PAGE gel of purified nanobodies (second purification attempt) | 1 |
| 11:50, 3 October 2022 | Nb1 cell assay.png (file) |  |
80 KB | Graph showing the inhibitory effect that anti-TNFa nanobody VHH#2B has on human monocytes stimulated with TNFa. | 1 |
