File list

This special page shows all uploaded files.

File list
 
Date Name Thumbnail Size Description Versions
13:00, 29 October 2017 Orthogonal Lox sites gel.png (file) 337 KB In vitro assay done by Edinburgh_UG 2017 to test function of orthogonal Lox sites. 1
21:38, 28 October 2017 Rox Corrected Assays.png (file) 67 KB All assays involving Rox target sites performed by Edinburgh_UG 2017. 1
19:59, 28 October 2017 VCre Corrected Assay.png (file) 62 KB All assays performed by Edinburgh_UG to assess VCre activity and cross-reactivity. 1
16:47, 28 October 2017 Edinburgh UG measurement constructs.png (file) 59 KB Schematic outlining principle behind all recombinase measurement constructs developed and tested by Edinburgh_UG 1
16:46, 28 October 2017 T7 Decreasing Leakiness.png (file) 64 KB Schematic outlining how leakiness can be decreased if two layers of induction are used with T7 RNA polymerase and an inducible T7 promoter. 1
14:24, 28 October 2017 Vox Assays.png (file) 96 KB All assays carried out by Edinburgh_UG on measurement constructs containing Vox Target Sites. 1
14:24, 28 October 2017 Vlox Assays.png (file) 72 KB All tests carried out by Edinburgh_UG on measurement constructs containing Vlox Target sites. 1
14:21, 28 October 2017 Vika assays.png (file) 75 KB All assays carried out by Edinburgh_UG involving inducibly-expressed recombinase Vika. 1
14:21, 28 October 2017 VCre Assays.png (file) 61 KB All assays carried out by Edinburgh_UG where listed measurement constructs were exposed to inducibly-expressed VCre recombinase. 1
14:20, 28 October 2017 Slox Assays.png (file) 70 KB All assays carried out by Edinburgh_UG involving target site Slox on one of our measurement constructs. 1
14:19, 28 October 2017 SCre Assays.png (file) 58 KB All assays carried out by Edinburgh_UG using induced SCre expression on our measurement constructs. 1
14:18, 28 October 2017 Rox Assays.png (file) 66 KB All assays carried out by Edinburgh_UG with measurement constructs containing Dre target site Rox. 1
14:17, 28 October 2017 Dre measurements.png (file) 64 KB All measurements taken with constructs containing Rox sites that were tested with cells inducibly expressing Dre recombinase. Dre recombinase was produced by incubating cells with IPTG. 1
14:16, 28 October 2017 20 Oct Assays.png (file) 88 KB Series of assays carried out by Edinburgh_UG on 20 October, 2017 1
14:12, 28 October 2017 17 October Series of Assays.png (file) 191 KB A series of measurements Edinburgh_UG carried out on 17 October 2017. 1
11:30, 28 October 2017 Sequencing Results Edinburgh UG.zip (file) 1.21 MB All successful sequencing reactions carried out by Edinburgh UG. They have been uploaded to all relevant parts pages. A "cipher" is included in the file to help understand labels on each file. All primers used were standard biobrick sequencing primer V... 1
12:04, 7 October 2017 BBa K2406053-graph 1.png (file) 36 KB Graph showing RFP expression as a result of Vika/Vox recombination causing excision of Terminator. Confirms function of Vika, Vox target sites, and T7-LacO promoter. 1
10:54, 7 October 2017 BBa K2406051-graph 1.png (file) 23 KB Graph comparing measurement construct fluorescent output when Dre recombinase production is induced with IPTG compared to control where no IPTG is added. Graph shows clear difference in fluorescence output, indicating inducible excision of terminator.... 1