File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 21:51, 31 October 2017 | 5plasmidresult.jpg (file) |  |
1.4 MB | SynORI 5 plasmid co-transformation results 1 - No trigger 1 (control). 2 - No trigger 2 (control). 3 - No lambda activator plasmid (control). 4 - Full System: lambda activator plasmid; toehold 1 alpha-neo; toehold 2 beta-neo; trigger 1; trigger 2. | 1 |
| 21:29, 31 October 2017 | 5plasmid.png (file) |  |
150 KB | The schematic representation of 5 plasmid SynORI selection system. First plasmid constantly expresses lambda, the activator of the modified phage promoter, which controls the expression of the Trigger 1 and 2. The Triggers unlock the translation of spl... | 1 |
| 21:14, 31 October 2017 | 4plasmid.png (file) |  |
80 KB | The schematic representation of 4 plasmid SynORI selection system. First 2 plasmids constantly express Toehold 1 and 2 which are coupled with the split antibiotic alpha and beta subunits but has its translation locked. Trigger 1 and 2 are constantly ex... | 1 |
| 17:13, 31 October 2017 | Alfabeta.jpg (file) |  |
4.05 MB | 2 vectors with constant expression of alpha and beta subunits were co-transformed into TG1 competent cells and plated on agar with kanamycin antibiotic | 1 |
| 12:09, 31 October 2017 | Toehold.png (file) |  |
25 KB | Toehold switches repress translation through base pairs programmed before and after the start codon (AUG), leaving the RBS and start codon regions completely unpaired. The toehold domain a binds to a complementary a* domain on the trigger RNA ant initi... | 1 |
