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Date Name Thumbnail Size Description Versions
18:08, 22 October 2016 AraC TOP10.png (file) 125 KB Characterisation of the pBAD-GFP construct with varying concentrations of L-arabinose. (BBa_K1893017).Experiments were performed in E. coli Top10 cell strain cultured at 37°C, which were diluted to 0.05 O.D. and inoculated with L-arabinose at the 0 mi... 1
18:07, 22 October 2016 Transfer curve arac.png (file) 26 KB Transfer function of normalised GFP fluorescence against concentration of L-arabinose 1
18:06, 22 October 2016 Gp2.png (file) 88 KB Growth inhibition of Top10 cells by induction of Gp2 by L-arabinose. Experiments were performed in E. coli Top10 cell strain cultured at 37°C, which were diluted to 0.05 O.D. and inoculated with L-arabinose at the 0 minute timepoint. O.D. was recorded... 1
18:02, 22 October 2016 Gp2 recovery.png (file) 68 KB Recovery of growth by TOP10 cells after arabinose-induced pBAD operon was switched off by glucose-mediated catabolite repression. 100uM of L-arabinose was added to the culture 2 hours before the 0 minute timepoint and D-glucose was added at the 0 minut... 1