File list
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| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 21:13, 12 October 2021 | Results influence of IPTG activation on the enzyme activity.png (file) |  |
217 KB | A +IPTG supernatant , +IPTG in vitro, +IPTG in vivo, −IPTG supernatant, −IPTG in vitro, −IPTG in vivo B Measurements: values for “in vitro” from experiment 1 and “in vitro + IPTG” differ noticeably even if experimental conditions were th... | 1 |
| 21:09, 12 October 2021 | Results β-agarase activity.png (file) |  |
222 KB | 2 | |
| 16:00, 12 October 2021 | Experimental setup agarase assay +IPTG.png (file) |  |
603 KB | overnight cultures of E. coli BL21 with pet15b-β-agarase and E. coli BL21 with pet15b-mcherry were grown at 37° in LB medium. To the overnight cultures as well as to the in vivo experiments, carbenicillin was added for selection and IPTG was added to... | 1 |
| 15:32, 12 October 2021 | Β-agarase pit formation.png (file) |  |
2.09 MB | Agarolytic activity was confirmed by pit formation on LB agar plates, 2 angles | 1 |
| 10:34, 9 October 2021 | Experimental setup agarase assay.png (file) |  |
509 KB | 1 | |
| 09:49, 7 October 2021 | 3,5-dinitrosalicylic acid method (Miller 1959).jpeg (file) | .jpeg){kind=small} |
32 KB | Chemical reaction of the 3,5-dinitrosalicylic acid (DNS) method (Miller 1959). Determination of Reducing Sugars | 1 |
| 15:03, 5 October 2021 | Figure1- Enzyme activity of the β-agarases.jpeg (file) |  |
81 KB | Enzyme activity of the β-agarases | 1 |
