File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 05:45, 8 October 2022 | 1hr absorbance.png (file) |  |
197 KB | 1 | |
| 11:25, 7 October 2022 | Final proof.png (file) |  |
37 KB | 1 | |
| 11:22, 7 October 2022 | Modeling table for OPH page.png (file) |  |
78 KB | 1 | |
| 11:10, 7 October 2022 | IPTG induction chart.jpg (file) |  |
184 KB | chart | 1 |
| 11:08, 7 October 2022 | IPTG induction (μM) vs. pNP concentration after 23 hours (μM).jpg (file) | _vs._pNP_concentration_after_23_hours_(%CE%BCM).jpg) |
71 KB | IPTG induction (μM) vs. pNP concentration after 23 hours (μM). After pNP concentration reaches a maximum at 250μM of IPTG induction, the amount of pNP will not increase as the concentration of IPTG increases. | 1 |
| 02:09, 6 October 2022 | The change in pNP concentration over 25 hours in culture.jpeg (file) |  |
66 KB | The change in pNP concentration over 25 hours in culture | 1 |
| 02:08, 6 October 2022 | Mechanism of polyP sensor.jpeg (file) |  |
113 KB | Mechanism of polyP sensor | 1 |
| 02:07, 6 October 2022 | Function of our biosensor upon IPTG induction (created by BioRender).jpeg (file) | .jpeg) |
188 KB | Function of our biosensor upon IPTG induction (created by BioRender) Our biosensor contains an enzyme plasmid and a sensor plasmid that would enhance GFP expression, thereby indicating the amount of paraoxon detoxified by OPH. | 1 |
| 02:07, 6 October 2022 | AsPhoU mechanic.jpeg (file) |  |
142 KB | PhoU protein function (left) and inhibition of PhoU by AsPhoU (right) | 1 |
| 02:00, 6 October 2022 | Presence of pNP.jpeg (file) |  |
47 KB | GFP fluorescence of DH5 alpha and DH5 alpha with biosensor in the absence/presence of pNP | 1 |
| 01:59, 6 October 2022 | Paraoxon degredation.jpeg (file) |  |
24 KB | Paraoxon degradation by OPH: The degradation of paraoxon into dimethyl phosphate (DMP) and p-nitrophenol (pNP) | 1 |
| 01:57, 6 October 2022 | PET22b plasmid.jpeg (file) |  |
225 KB | PET22b plasmid | 1 |
| 01:57, 6 October 2022 | AsPhoU cut with NcoI and XhoI.jpeg (file) |  |
315 KB | AsPhoU cut with NcoI and XhoI | 1 |
| 01:55, 6 October 2022 | OPH cut.jpeg (file) |  |
419 KB | Gel electrophoresis of pET22b::OPH after digested with BamHI and HindIII respectively Column 2 shows the result of pet22b::OPH digested by BamHI while column 3 shows the result of pET22b::OPH digested by HindIII. Both show 6.5 kilobases of linear DNA. | 3 |
| 01:54, 6 October 2022 | OPH BamHI and HindiIII combined digestions.jpeg (file) |  |
324 KB | The 7th column shows the result of restriction enzyme digestion by BamHI and HindIII; the DNA bands include pET22b::OPH, OPH (1071 bases), and pET22b vector (5479 bases). | 1 |
| 12:16, 5 October 2022 | BBa J18932 mCherry.png (file) |  |
22 KB | mCherry RFP Red fluorescent protein derived from DsRed detected at λ (excitation) = 415 nm and λ (emission) = 550 nm | 1 |
| 12:11, 5 October 2022 | BBa K4271014 PolyP sensor without SsrA degradation tag.png (file) |  |
61 KB | BBa_K4271014 PolyP sensor without SsrA degradation tag | 1 |
| 12:10, 5 October 2022 | BBa K4271013 PolyP sensor with SsrA degradation tag.png (file) |  |
68 KB | BBa_K4271013 PolyP sensor with SsrA degradation tag that enhances the rate of fluorescent protein degradation so that the protein would not accumulate and generate false signals for detection. | 1 |
| 12:08, 5 October 2022 | BBa K4271011 ureA promoter.png (file) |  |
28 KB | BBa K4271011 ureA promoter.png | 1 |
| 11:46, 5 October 2022 | BBa K4271010 rpoD sigma factor.png (file) |  |
23 KB | BBa_K4271010 rpoD sigma factor | 1 |
| 11:45, 5 October 2022 | BBa K4271009 Tac promoter.png (file) |  |
29 KB | BBa_K4271009 Tac promoter | 1 |
| 11:43, 5 October 2022 | BBa K4271007 pobR operator.png (file) |  |
15 KB | BBa_K4271007 pobR operator | 1 |
| 11:43, 5 October 2022 | BBa K4271006 pNP RBS.png (file) |  |
14 KB | BBa_K4271006 pNP RBS | 1 |
| 11:43, 5 October 2022 | R dual directional promoter.png (file) |  |
19 KB | 1 | |
| 11:42, 5 October 2022 | BBa K4271004 pNPmut1-1.png (file) |  |
12 KB | BBa_K4271004 pNPmut1-1 | 1 |
| 11:41, 5 October 2022 | BBa K4271003 AsPhoU Composite.png (file) |  |
48 KB | BBa_K4271003 AsPhoU Composite | 1 |
| 11:40, 5 October 2022 | BBa K4271016 AsPhoU RBS.png (file) |  |
16 KB | 1 | |
| 11:39, 5 October 2022 | BBa K4271002 Antisense-PhoU (AsPhoU).png (file) | .png) |
12 KB | 1 | |
| 11:38, 5 October 2022 | BBa K4271015 pelB signal peptide.png (file) |  |
21 KB | 1 | |
| 11:36, 5 October 2022 | BBa K4271001 OPH Composite.jpeg (file) |  |
22 KB | 1 | |
| 04:49, 1 October 2022 | BBa K4271000.png (file) |  |
19 KB | 1 | |
| 04:47, 1 October 2022 | Linear map pNP sensor.png (file) |  |
86 KB | 1 |
