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Date Name Thumbnail Size Description Versions
23:42, 21 October 2021 T--USP-Brazil--IGEM-USP-Description-Fusionedcyp2.png (file) 160 KB   1
23:25, 21 October 2021 T--USP-Brazil--IGEM-USP-Description-Fusionedcyp1.png (file) 182 KB Schematic representation of the P450-redox partner interaction. P450, cytochrome 450 monooxygenase; CPR, cytochrome P450 reductase; FAD, flavin adenine dinucleotide; FMN, flavin mononucleotide; NADPH, reduced nicotinamide adenine dinucleotide phosphate. 1
19:25, 21 October 2021 T--USP-Brazil--IGEM-USP-Description-CYP6g1Model.png (file) 94 KB   1
19:24, 21 October 2021 File-File-T--USP-Brazil--IGEM-USP-Description-GUSpollen.png (file) 317 KB Figure 1. Presence of GUS activity on plants. (-C) - Pollen negative control. (+C) - Leaf positive control (GUS expressed by CaMV35S promoter). 2 and 22 - Transformants Micro-tom for our circuit. Blue pollen cells are indicated by arrows in pictures 2... 1
19:24, 21 October 2021 File-T--USP-Brazil--IGEM-USP-Description-qRTPCRtable.png (file) 32 KB   1
19:18, 21 October 2021 T--USP-Brazil--IGEM-USP-Description-Lat52GUS.png (file) 108 KB Figure 2. Schematic representation for β-glucuronidase (GUS) assay on pollen. The catalysis of X-Gluc produces a chromophore that can be easy to visualize and shows the traduction of transcript. 1
18:36, 21 October 2021 T--USP-Brazil--IGEM-USP-Description-Lat52 Overall.png (file) 84 KB Figure 1.Scheme of steps for RT-qPCR. RNA from leaf and pollen are extracted and purified, then the samples are used as template for cDNA synthesis. Next, the standardized cDNA was used to perform the qRT-PCR to prove the difference of transcripts rate. 1