Ribosome Binding Sites/Prokaryotic/Constitutive/Miscellaneous

In addition to the RBS families listed above, the registry has a collection of miscellaneous constitutive prokaryotic RBSs. These RBSs are typically designed for a specific application or correspond to the natural RBS used by a particular gene in an organism of interest. While these RBS are likely to work for gene expression in E. coli, we recommend using the families described above as there is typically more data on their behavior and they have been shown to work by multiple groups. The miscellaneous collection also includes some RBS designed to work for genes coded on the reverse DNA strand.


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NameSequenceDescriptionRelative
Strength		Predicted
Strength
BBa_B0036gtgtgSpecialized RBS  
BBa_B0037gtgtgtctagSpecialized RBS  
BBa_B0038tcacacaggaaaccggttcgatgRBS 1  
BBa_B0039tcacacaggaaaggcctcgatgRBS 2  
BBa_B0041tcacacaggacggccggatgRBS 3  
BBa_B0070tctcacgtgtgtcaagSpecialized RBS modified from that of B0036 (Brink et al.)  
BBa_B0071tctcacgtgtgtSpecialized RBS  
BBa_B0076catccctSpecialized RBS  
BBa_B0077tcacatccctSpecialized RBS  
BBa_B0078tcacatccctccSpecialized RBS  
BBa_B2001actgcacgaggtaacacaagT7 RBS 0.3   
BBa_B2002tacgaggaggatgaagagtaT7 RBS 0.4  
BBa_B2003actttacttatgagggagtaT7 RBS 0.5  
BBa_B2017acgaagacggagacttctaa2.8 RBS from T7  
BBa_B2022aaccctcaggaggtaaacca4B RBS from T7  
BBa_B2040aagacatggagacacattta8 RBS from T7  
BBa_B2101actgcacgaggtaacacaagT7 RBS 0.3 + SapI (rev)  
BBa_B2102 . . . gaggaggatgaagagtaatgtgaagagctgT7 RBS 0.4 + SapI (rev)  
BBa_I11010aggaggtcatcRBS  
BBa_I723012gcaagctcttttttcagttgtctcEstimated RBS for DntR  
BBa_I723014ctgatagttaaaatcaccagcatgaEstimated RBS for DntA  
BBa_I723019taaaaacaagaggaaaacaaRBS for XylR  
BBa_I742130tctcctctttReverse RBS  
BBa_I742145acggagaagcagcgaasam5 native rbs  
BBa_I742146gaggttgggacaagsam8 native rbs  
BBa_I742150 . . . taaatgtatccgtttataaggacagcccgacrtE native rbs  
BBa_I742153ctcttaagtgggagcggctcrtY native rbs  
BBa_I742156ctctaccggagaaattcrtZ native rbs  
BBa_I742159ctcatcgttaaagagcgactaccrtI native rbs  
BBa_I742163ctcagcctgtacctggagagcctttcnative ftsK rbs  
BBa_J15001ctcaaggaggstrong synthetic E. coli ribosome binding site with SacI site.  
BBa_J26002gagaggMario Binding Site   
BBa_J29048aggaggattacaaRBS  
BBa_J34801aaagaggagaaaribosome binding site  
BBa_J34803tcacacaggaaagribosome binding site  
BBa_J34810ggaagaggribosome binding site  
BBa_J44001tttctcctctttaatReverse RBS (RBSrev) -- corresponds to BBa_B0030n/a 
BBa_J56013tcacacaggaaaggcctcgRbs2 ribosome binding site   
BBa_J56016attaaagaggagaaattaagcRbs-orig - ribosome binding site  
BBa_J59001 . . . tcgtttctgaaaaattttcgtttctgaaaatuba  
BBa_J61140tggctaacatagggt{rbs1} Library Member in BBb  
BBa_J61141tggctaactgaggat{rbs1} Library Member in BBb  
BBa_J61142tggctaacccagggt{rbs1} Library Member in BBb  
BBa_J61143tggctaactcaggtg{rbs1} Library Member in BBb  
BBa_J61144tggctaaccctggta{rbs1} Library Member in BBb  
BBa_J61145tggctaacttgggac{rbs1} Library Member in BBb  
BBa_J61146tggctaacgcaggtc{rbs1} Library Member in BBb  
BBa_J61147tggctaacatcggtg{rbs1} Library Member in BBb  
BBa_J64011ttaattaaggaaaagatctinvB RBS  
BBa_J64013cagaagaggatattaatasipA RBS  
BBa_J64015ttgataaggaattgtasopA RBS  
BBa_J64018tcagaggagataatttainvJ RBS  
BBa_J64020tgacacgttgagcggtatgainvI RBS  
BBa_J64022acagataacaggagtaagtasicA RBS  
BBa_J64024taaagggagaaaaatsipC RBS  
BBa_J64026gagtcttgaggtaactatsigE RBS  
BBa_J64028tcaggaatattaaaaacgctsopB RBS  
BBa_J64030atttgaaggaaaatattsopD RBS  
BBa_J64031caaaaacatactgcaggaatsptP RBS  
BBa_J64609tgccattgcaaaggagaagactcreD RBS  
BBa_J64807aagggggaattcaaatRocD RBS  
BBa_J64808aaggggtgcagaatRocE RBS  
BBa_J64809aggtggaatcacagRocF RBS  
BBa_J64907atagataaaaatggtaacaatcreA RBS  
BBa_J64908gggatatagcctgaggggcctgtaRBS for creC in e coli operon  
BBa_J64968cggcaataacagaggcgatttRBS on creB  
BBa_K082000attaaagaggagaaatareconstruct strong RBS  
BBa_K082001tcacacaggaaagtaRBS medium  
BBa_K090505aaaggaggtgt''Bacillus subtilis'' consensus RBS  
BBa_K090506agaggtggtgt''Bacillus subtilis'' weak RBS  
BBa_K103015aggaggconsensus RBS  
BBa_K118012gaggSynthetic ribosome binding site added by Son of Babel procedure  
BBa_K143020taaaggaggaaGsiB ribosome binding site (RBS) for B. subtilis  
BBa_K143021aaaggtggtgaaSpoVG ribosome binding site (RBS) for B. subtilis  
BBa_K150005aggaaacagaaccribosome binding site of pTrc99a  
BBa_K4382009aaggaggaaggatcaRBS for Bacillus Subtilis  
BBa_K4462010ttataaggaggtRBS.15  
BBa_K780001atattaagaggaggagStrong RBS for Bacillus Subtilis  
BBa_K780002agagaacaaggaggggStrong RBS for Bacillus Subtilis  
BBa_M13501gattgggataaataatM13K07 gene I RBS  
BBa_M13502atcaaccggggtacatM13KO7 gene II RBS  
BBa_M13503tttggagattttcaacM13K07 gene III RBS  
BBa_M13504aaaaaaggtaattcaaM13K07 gene IV RBS  
BBa_M13505cataaggtaattcacaM13KO7 gene V RBS  
BBa_M13506ataaggagtcttaatcM13K07 gene VI RBS  
BBa_M13507gttccggctaagtaacM13KO7 gene VII RBS  
BBa_M13508taatggaaacttcctcM13K07 gene VIII RBS  
BBa_M13509tcgctgggggtcaaagM13Ko7 gene IX RBS  
BBa_M13510atttgagggggattcaM13KO7 gene X RBS  
BBa_M13511aatttaggtcagaagM13K07 gene XI RBS  
BBa_Z0261aatcaataggagaaatcaatStrong T7.2 RBS  
BBa_Z0262ttaaagaggagaaatactagMedium strength T7.2 RBS