Protein coding sequences/Biosynthesis/AHL
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N-Acyl Homoserine lactones (AHLs or N-AHLs) are a class of signaling molecules involved in bacterial quorum sensing. Quorum sensing is a method of communication between bacteria that enables the coordination of group based behavior based on population density. In synthetic biology, genetic parts derived from quorum sensing systems have been used to create patterns on a lawn of bacteria and to achieve synchronized cell behavior. AHL can diffuse across cell membranes and is stable in growth media over a range of pH values. AHL can bind to transcriptional activators such as LuxR and stimulate transcription from cognate promoters. Several similar quorum sensing systems exists across different bacterial species; thus, there are several known enzymes that synthesize or degrade different AHL molecules. Note that genetic parts derived from different quorum sensing systems may have different levels of crosstalk.
Biosynthesis
| Name | Protein | Description | Direction | Uniprot | KEGG | E.C. | Substrate | Product | Length | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| BBa_C0060 | aiiA-LVA | autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone | Forward | Q1WNZ5 | none | 3.1.1.- | 814 | In stock | ||
| BBa_C0061 | luxI-LVA | autoinducer synthetase for AHL | Forward | P12747 | none | none | 643 | In stock | ||
| BBa_C0070 | rhlI-LVA | autoinducer synthetase for N-butyryl-HSL (BHL) and HHL | Forward | Q02QW5 | none | none | 667 | In stock | ||
| BBa_C0076 | cinI | autoinducer synthetase | Forward | Q1MDW1 | none | none | 727 | In stock | ||
| BBa_C0078 | lasI | autoinducer synthetase for PAI from Pseudomonas aeruginosa | Forward | P33883 | pae:PA1432 | none | 667 | In stock | ||
| BBa_C0161 | luxI | autoinducer synthetase for AHL (no LVA) | Forward | P12747 | none | none | 585 | In stock | ||
| BBa_C0170 | rhII | autoinducer synthetase for N-butyryl-HSL (BHL) and HHL (no LVA) | Forward | Q02QW5 | none | none | 609 | In stock | ||
| BBa_C0178 | lasI | autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA) | Forward | P33883 | pae:PA1432 | none | 609 | In stock | ||
| BBa_K091109 | LuxS | 516 | In stock | |||||||
| BBa_K1060000 | AroG; DAHP synthase | 1053 | In stock | |||||||
| BBa_K2656019 | LuxI Coding Sequence | 586 | It's complicated |
Degradation
| Name | Protein | Description | Direction | Uniprot | KEGG | E.C. | Substrate | Product | Length | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| BBa_C0060 | aiiA-LVA | autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone | Forward | Q1WNZ5 | none | 3.1.1.- | 814 | In stock | ||
| BBa_C0160 | aiiA | autoinducer inactivation enzyme aiiA (no LVA) | Forward | Q1WNZ5 | none | 3.1.1.- | 756 | In stock |