Coding

Part:BBa_M45090:Design

Designed by: Yonas Kidane   Group: UtahState_BE5930_-_S14   (2014-04-15)

CUP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

    Amplification of a particular gene is one of the few mechanisms to achieve increased production of certain 

macromolecules in the cells. In this study several genes has been used as binding protein (list of the gene can be

found in the this journal- Eapen and Souza, 2005). As an example we will discuss on CUP1 which is one of the metal

binding proteins for cadmium. CUP1 gene is obtained from NCBI website (NM_011791854). The restriction sites of the 

gene were removed so as to avoid the digestion of DNA. The gene is then amplified by PCR.

    The linear DNA containing CUP1 gene of interest is transferred to the chromosome by Homologous recombination and

the yeast strain is selected for growth (Tauseef and David, 1987). After the metal-binding protein is synthesized, it

was expressed as a fusion to the surface of exposed area of the membrane protein. This provides the yeast cell with 

higher capacity to accumulate Cadmium metals.

Source

The souce is obtained from NCBI website with reference NM_011791854

References

Susan Eapen , S.F. D’Souza. Prospects of genetic engineering of plants for phytoremediation of toxic metals. Biotechnology Advances 23 (2005) 97– 114.

Tauseef R. Butt, David J. Ecker. Yeast Metallothionein and Applications in Biotechnology. MICROBIOLOGICAL REVIEWS, Sept. 1987. p. 351-364.