Composite

Part:BBa_M36953:Experience

Designed by: Amiel Stephen Paz, William Kindschuh, Vickram Gidwani   Group: Stanford BIOE44 - S11   (2015-10-24)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36953

For a study on the potentials of RiAFP as a cryoprotectant, the part was inserted into a vector backbone consisting of a constitutive promoter and GFP reporter protein and subsequently transfected within a HEK293T cell. Below is the flow cytometry data concluding aspects of the transfected (and non-transfected) samples' cell viabilities and overall transfection efficiency.

Performance Data

http://i.snag.gy/sSGLa.jpg


http://i.snag.gy/9wZR9.jpg


http://i.snag.gy/YjWFa.jpg


Stanford Location

Plasmid 1: pRiAFP1, Gene #: 232008, E. coli, actuator, barcode #: 133018648, BIOE44 F15 Box 2

Plasmid 2: pRiAFP2, Gene #: 232009, E. coli, actuator, barcode #: 133027061, BIOE44 F15 Box 2

User Reviews

UNIQdc1aad293b1727a9-partinfo-00000000-QINU UNIQdc1aad293b1727a9-partinfo-00000001-QINU