Device

Part:BBa_M36279:Experience

Designed by: Victoria Robles, Kyung Ri Park, Ken Xiong   Group: Stanford BIOE44 - S11   (2011-12-09)

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Applications of BBa_M36279

This construct could not be expressed successfully in E. coli (DH105) using a high copy version of the Gemini plasmid for reasons unknown to us. However, DNA 2.0 was able to provide us with a modified version that could be expressed in one of their in-house plasmids. Unfortunelatey, the modification included a 32 basepair deletion from 851-882bp, which is most of the copYAZ promoter. The modified construct did not behave as expected to none of our surprise. When the sensor was linked to the Gemini reporter (Endy 2009), we found that exposing transformed cells to increasing levels of copper did not induce a significant expression of Gemini.

User Reviews

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