Composite

Part:BBa_K953003:Design

Designed by: David Dubow   Group: iGEM12_Macquarie_Australia   (2012-09-25)


Agrobacterium tumefaciens Bacteriophytochrome with T7 & RBS (E. coli Codon Optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1559
    Illegal BamHI site found at 1919
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for E. coli, whilst staying within the GC constraints of gibson assembly.


Source

Gibson Assembly

References