Coding

Part:BBa_K808026:Design

Designed by: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling   Group: iGEM12_TU_Darmstadt   (2012-09-02)


pNB-Est 13: Esterase PET cleaving enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1071
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Our team obtained it from Henkel GmbH on an pET26b(+) vector: Fakult, M., & Enzymtechnologie, M. (2006). Isolierung, Identifizierung und biochemische Charakterisierung Dialkylphthalat spaltender Esterasen.

References