Part:BBa_K676778 Deleted

Extractery

Usage and Biology

DNA gyrase is the only topoisomerase that introduces negative supercoiling into closed circular plasmid molecules. It is a heterotetramer consists of two GyrA and two GyrB subunits.

This expression cassette is regulated by an IPTG inducible promoter and will be expressing both Gyr A and Gyr B subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. Gyr B subunit binds to ATP which will provide energy for the progress of catalytic reaction in the presence of divalent cations such as magnesium cation.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3932
  Illegal PstI site found at 1424
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1247
  Illegal NheI site found at 3558
  Illegal PstI site found at 1424
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 867
  Illegal BglII site found at 2329
  Illegal BglII site found at 4307
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3932
  Illegal PstI site found at 1424
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3932
  Illegal PstI site found at 1424
  Illegal NgoMIV site found at 1033
  Illegal AgeI site found at 2975
  Illegal AgeI site found at 3045
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1520