Translational_Unit
HO1

Part:BBa_K646000:Design

Designed by: Edward Moh   Group: iGEM11_Macquarie_Australia   (2011-09-29)

Hemeoxygenase with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was digested with XbaI and SpeI for the BioBrick construction due to the primer design, and the gene orientation was screened by an XbaI digest of the constructed BioBrick. For subsequent usage of this part, iGEM BioBrick assembly protocol could be used; digest using either EcoRI with SpeI or XbaI with PstI.

Source

Http://www.ncbi.nlm.nih.gov/gene/953483

References