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Reporter

Part:BBa_K641002:Design

Designed by: Spencer Scott   Group: iGEM11_Berkeley   (2011-08-31)


{Pctx}{rbs.ffGFP}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The only design considerations were tuning an rbs to the ffGFP so that expression levels could clearly be distinguished.


Source

Pctx is a promoter from a virulence island in Vibrio cholerae. The ffGFP is a widely used purely synthetic protein.

References