Generator

Part:BBa_K581014:Design

Designed by: Weiyue Ji, Dingqiao Wen   Group: iGEM11_Peking_S   (2011-09-26)


lux repressible promoter-rbs-GFP-ssrA-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 953
    Illegal SpeI site found at 808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 808
    Illegal SpeI site found at 954
    Illegal PstI site found at 968
    Illegal NotI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 954
    Illegal SpeI site found at 808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 808
    Illegal SpeI site found at 954
    Illegal PstI site found at 968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


Design Notes

The part can demonstrate the effectiveness of the lux_invert promoter.


Source

They are assembled from basic parts in the Registry.

References