Composite
Part:BBa_K5519004
Designed by: Latifat Abdulsalam Group: iGEM24_KFUPM-Saudi-Arabia (2024-09-16)
AB_pTac_BborAMP1_TEVcut_GFP_His
This is a composite part containing a novel antimicrobial peptide gene gotten from the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This composite part is made up of an AB_pTac promoter, which does not require any specific RNA polymerase, a BCB12 RBS, BborAMP1 (the coding sequence), which is the novel AMP gene attached to a TEVcut site, GFP as a reporter gene and a His-tag protein for easy purification and detection of the AMP by immobilized metal affinity chromatography (IMAC).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1093
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1093
Illegal NotI site found at 1235 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1093
Illegal BamHI site found at 1087
Illegal XhoI site found at 1244 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1093
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1093
Illegal NgoMIV site found at 1018
Illegal AgeI site found at 487 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |