Composite

Part:BBa_K5519004

Designed by: Latifat Abdulsalam   Group: iGEM24_KFUPM-Saudi-Arabia   (2024-09-16)


AB_pTac_BborAMP1_TEVcut_GFP_His

This is a composite part containing a novel antimicrobial peptide gene gotten from the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This composite part is made up of an AB_pTac promoter, which does not require any specific RNA polymerase, a BCB12 RBS, BborAMP1 (the coding sequence), which is the novel AMP gene attached to a TEVcut site, GFP as a reporter gene and a His-tag protein for easy purification and detection of the AMP by immobilized metal affinity chromatography (IMAC).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1093
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1093
    Illegal NotI site found at 1235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1093
    Illegal BamHI site found at 1087
    Illegal XhoI site found at 1244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1093
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1093
    Illegal NgoMIV site found at 1018
    Illegal AgeI site found at 487
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None