Coding

Part:BBa_K5492202:Design

Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka   Group: iGEM24_TERMOSZ-Selye-HUN   (2024-09-15)


Wild type rDAO - pETDUET-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal BglII site found at 1050
    Illegal BglII site found at 2303
    Illegal BamHI site found at 1978
    Illegal BamHI site found at 2232
    Illegal XhoI site found at 848
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.

Source

The work of iGEM20_SEHS-China (BBa_K3684002) provided the dna sequence of rDAO.

References

https://www.twistbioscience.com/faq/gene-synthesis/what-are-adapter-sequences-used-adapter-gene-fragments