Regulatory

Part:BBa_K549038:Design

Designed by: Jara Radeck   Group: iGEM11_LMU-Munich   (2011-09-21)


Promotor prcnA of rcnA with RcnR-binding site and 50nt rcnA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter contains the first ca. 50 nt of rcnA, and therefore is used for transcriptional fusion with genes with RBS. This BB can be used for measurement of Ni(II)-concentration.


Source

It originates from E.coli K12 MG 1655 gDNA.

References