Part:BBa_K5477021

CYP3A4-MYC - Cytochrome P450 3A4 codon-optimized with MYC tag for Saccharomyces cerevisiae

CYP3A4-MYC refers to a variant of the CYP3A4 enzyme that has been tagged with a MYC epitope. The MYC tag is a short peptide sequence derived from the c-MYC oncoprotein, and it is widely used to facilitate the detection, purification, or localization of tagged proteins using anti-MYC antibodies. In the case of CYP3A4-MYC, the CYP3A4 enzyme retains its natural function as a cytochrome P450 enzyme, responsible for the oxidation and metabolism of a wide range of substrates, including pharmaceuticals, environmental toxins, and endogenous compounds (1) (2) (3). The addition of the MYC tag allows to easily detect and quantify CYP3A4 expression levels in cells. CYP3A4-MYC would provide the same broad detoxification functions as untagged CYP3A4, initiating the oxidative metabolism of drugs, pollutants, and toxins (3).

In the context of our system, CYP3A4-MYC is integrated into a detoxification module where it initiates the oxidative breakdown of toxic compounds, such as pharmaceuticals and environmental pollutants, allowing these substances to be further processed and excreted. This part was combined with POR. The following composites and devices, where this part was used, are listed below:

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1483
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1540
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1483
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1483
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Guengerich, F.. (2008). Cytochrome P450 and Chemical Toxicology. Chemical research in toxicology. 21. 70-83. 10.1021/tx700079z.

2. Klyushova LS, Perepechaeva ML, Grishanova AY. The Role of CYP3A in Health and Disease. Biomedicines. 2022 Oct 24;10(11):2686. doi: 10.3390/biomedicines10112686. PMID: 36359206; PMCID: PMC9687714.

3. Lynch T, Price A. The effect of cytochrome P450 metabolism on drug response, interactions, and adverse effects. Am Fam Physician. 2007;76(3):391-396.