Composite

Part:BBa_K5422011:Design

Designed by: HOAREAU Romain   Group: iGEM24_Aix-Marseille   (2024-09-25)


Dual Protein Expression System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 709


Design Notes

During the design of this sequence, we optimized the codons for efficient expression in E. coli and ensured compatibility with BioBrick and Type IIS assembly standards. Additionally, we made adjustments to prevent secondary structures that could affect transcription and translation efficiency, and to facilitate the synthesis process by IDT. Importantly, these modifications involved synonymous codon changes, ensuring no alteration in the amino acid sequence.


Source

The DNA sequence for this part was synthesized by Integrated DNA Technologies (IDT) based on a custom design tailored for our project specifications.

References