Part:BBa_K538201:Design

pLacI + RBS + Cpn60

LacI:Cpn60 K538201

This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the Lac operon's promoter (pLacI: BBa_R0010) so that it may be expressed constitutively in LacI-null strains, or inducibly in LacI-capable strains. This promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538001.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.

• BBa_R0010
  • Main Page
  • Design Page
• BBa_B0034
  • Main Page
  • Design Page
• BBa_K538001
  • Main Page
  • Design Page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1096
  Illegal BamHI site found at 1162
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]