Composite

Part:BBa_K5366024:Design

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)


T7 promoter-RBS-TET-6xHis-T7 termonator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal NheI site found at 1564
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal BglII site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 64
    Illegal EcoRI site found at 199
    Illegal PstI site found at 103
    Illegal PstI site found at 748
    Illegal PstI site found at 1513
    Illegal NgoMIV site found at 795
    Illegal AgeI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured that the forward and reverse primer had 20 bp of homology to the plasmid.We needed to purify the TET protein, so we attached the 6×His tag after TET.

Source

Candidatus Aerophobetes bacterium

References