Coding

Part:BBa_K5325003:Design

Designed by: Hien Le, Dang Minh Anh   Group: iGEM24_MichiganState   (2024-10-01)


Pa_TB13_plaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of the FLAG tag that aids protein purification and visualization on Western Blot.

DNA sequence was codon optimized for expression in E. coli and Shewanella oneidensis.

Source

The part originated from the plaA gene of Paenibacillus amylolyticus.
NCBI ID and link to NCBI profile: AB093482.1

References

Akutsu-Shigeno, Y., Teeraphatpornchai, T., et al. (2003). Cloning and Sequencing of a Poly( dl -Lactic Acid) Depolymerase Gene from Paenibacillus amylolyticus Strain TB-13 and Its Functional Expression in Escherichia coli. Appl Environ Microbiol. 69(5):2498-2504.