Composite

Part:BBa_K5311013

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-26)


MG_026 + RBS cell free MJ + Cry4Ba + RoxP + His-tag

Genetic construct created to produce Cry toxin 4Ba constitutively in C. acnes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1845
    Illegal BglII site found at 3075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 607
    Illegal NgoMIV site found at 3566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1676
    Illegal BsaI.rc site found at 3779


[edit]
Categories
Parameters
None