Part:BBa_K5302018

pBBR-OmpA-8IJZ

8IJZ is an anti-VEGF nanobody, and it has 2 chains, chainA sequence: mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggykyd avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. chainB sequence:mdvqlvesgg glvqpggslr lscaasgrtf ssysmgwfrq apgkerefvv aiskggyky avslegrfti srdnakntvy lqinslrped tavyycassr aygssrlrla dtyeywgqgt lvtvss. These two chains all show great affinity with VEGF, we used pBBR1MCS-2 plasmid as a backbone and transfered 8IJZ into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IJZ.

Figure 1. Colony PCR results of pBBR-INP-8IJZ

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8IJZ is an anti-VEGF nanobody, and it has 2 chains.These two chains all show great affinity with VEGF, we plan to use pBBR1MCS-2 plasmid as a backbone and transfer 8IJZ into Escherichia coli Nissle 1917, and finally succeeded in expressing 8IJZ.

Figure 2. structure of 8IJZ， from PDB

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1920
  Illegal XbaI site found at 3081
  Illegal PstI site found at 2188
  Illegal PstI site found at 3093
  Illegal PstI site found at 4254
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1920
  Illegal PstI site found at 2188
  Illegal PstI site found at 3093
  Illegal PstI site found at 4254
  Illegal NotI site found at 5211
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1920
  Illegal BglII site found at 4467
  Illegal BamHI site found at 3075
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1920
  Illegal XbaI site found at 3081
  Illegal PstI site found at 2188
  Illegal PstI site found at 3093
  Illegal PstI site found at 4254
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1920
  Illegal XbaI site found at 3081
  Illegal PstI site found at 2188
  Illegal PstI site found at 3093
  Illegal PstI site found at 4254
  Illegal NgoMIV site found at 1262
  Illegal NgoMIV site found at 3520
  Illegal NgoMIV site found at 3803
  Illegal AgeI site found at 1422
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4891
  Illegal SapI site found at 3743
  Illegal SapI site found at 3953