Composite

Part:BBa_K5301019:Design

Designed by: Xing Zhang   Group: iGEM24_BNU-China   (2024-09-24)


MSP1E3D1 expression pathway (T7)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 173
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 173
    Illegal BglII site found at 799
    Illegal XhoI site found at 695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lac operator helps it to be expressed in T7 system.


Source

It's genetically engineered to produce nanodiscs better.

References