Coding

Part:BBa_K5301016:Design

Designed by: Yiran Cheng   Group: iGEM24_BNU-China   (2024-09-23)


PEG10 UTR flanked-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 307
    Illegal BsaI.rc site found at 147
    Illegal SapI.rc site found at 370


Design Notes

The length of PEG10 UTR sequence is flexible, and in our cell environment, about 500bp of PEG10 3'UTR work best.


Source

https://www.ncbi.nlm.nih.gov/gene/23089


References

[1] Segel, M., et al., Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery. Science, 2021. 373(6557): p. 882-889.