Coding

Part:BBa_K5301003:Design

Designed by: Xiaowen Sun   Group: iGEM24_BNU-China   (2024-09-22)


SpyTag can achieve covalent binding of proteins through Tag-Catcher interaction.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

SpyTag comes from the spontaneous isopeptide bond domain in streptococcus pyogenes fibronectin-binding protein FbaB.

References