Primer
Part:BBa_K5232000
Designed by: Kota Ishizuka Group: iGEM24_UTokyo (2024-09-27)
3step-SDA template 1
The 3' end side has a sequence complementary to primer0 amplified by the TWJ-SDA reaction ^[1], and the 5' end side is the template strand for primer1 ^[2]. <img src="https://static.igem.wiki/teams/5232/wiki/mechanism/mechanism-twj-3step-ds.svg"alt="hogehoge"> <img src="https://static.igem.wiki/teams/5232/wiki/result/result-b1-twj-123-newtemplate.svg" alt="hogehoge"> When TWJ-SDA was connected to 3step-SDA, an increase in amplification speed was observed around the 40-minute mark.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |