Primer

Part:BBa_K5232000

Designed by: Kota Ishizuka   Group: iGEM24_UTokyo   (2024-09-27)


3step-SDA template 1

The 3' end side has a sequence complementary to primer0 amplified by the TWJ-SDA reaction ^[1], and the 5' end side is the template strand for primer1 ^[2]. <img src="https://static.igem.wiki/teams/5232/wiki/mechanism/mechanism-twj-3step-ds.svg"alt="hogehoge"> <img src="https://static.igem.wiki/teams/5232/wiki/result/result-b1-twj-123-newtemplate.svg" alt="hogehoge"> When TWJ-SDA was connected to 3step-SDA, an increase in amplification speed was observed around the 40-minute mark.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None