Part:BBa_K5213000:Design

rboh [Chondrus crispus]

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2213
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2161
  Illegal AgeI site found at 2431
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1893
  Illegal SapI site found at 1926
  

Design Notes

Codon harmonized for expression in Cupriavidus necator (C. necator); stop codon changed to TAATAA

Source

Chondrus crispus [ORGN], DQ131542 [GenBank nucleotide ID], AAZ73480 [GenBank protein ID],

Herve et al. (2006) - NADPH oxidases in Eukaryotes: red algae provide new hints!

Thapa et al. (2020) - Genetic and biochemical reconstitution of bromoform biosynthesis in Asparagopsis lends insights into seaweed ROS enzymology

References

[1]C. Hervé, T. Tonon, J. Collén, E. Corre, and C. Boyen, “NADPH oxidases in Eukaryotes: red algae provide new hints!,” Curr Genet, vol. 49, no. 3, pp. 190–204, Mar. 2006, doi: 10.1007/S00294-005-0044-Z.

[2]H. R. Thapa, Z. Lin, D. Yi, J. E. Smith, E. W. Schmidt, and V. Agarwal, “Genetic and biochemical reconstitution of bromoform biosynthesis in Asparagopsis lends insights into seaweed ROS enzymology,” ACS Chem Biol, vol. 15, no. 6, p. 1662, Jun. 2020, doi: 10.1021/ACSCHEMBIO.0C00299.