Composite

Part:BBa_K5187009

Designed by: Changqi Fan   Group: iGEM24_Tsinghua   (2024-09-23)


Tetrathionate Sensing Colonization System

We selected the signaling pathway of the sensor, designed by a previous team, that can detect tetrathionate (a marker of IBD) and we connected the ALS3 adhesion protein downstream. When the system detects the marker of IBD, Saccharomyces cerevisiae can adhere to the intestinal tract by expressing the ALS3 protein, achieving the colonization of the engineered strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1509
    Illegal NgoMIV site found at 2953
    Illegal AgeI site found at 641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 358
    Illegal BsaI.rc site found at 3118
    Illegal BsaI.rc site found at 3157


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