Regulatory

Part:BBa_K5187002

Designed by: Changqi Fan   Group: iGEM24_Tsinghua   (2024-09-23)


pFUS1: Downstream promoter for our artificial signaling pathway

When our MOR215 receptor receives the muscone signal, it triggers the mating pathway in yeast through the modified Gα receptor, and pFUS1 is the downstream promoter of this signaling pathway. We achieved muscone-induced gene expression by constructing the pFUS1 promoter into a plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 538
    Illegal PstI site found at 1371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 538
    Illegal PstI site found at 1371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 538
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 538
    Illegal PstI site found at 1371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 538
    Illegal PstI site found at 1371
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None