Protein_Domain

Part:BBa_K5187001

Designed by: Changqi Fan   Group: iGEM24_Tsinghua   (2024-09-23)


GPA1-GNAI3: Synthetic Gα protein that responds to MOR215-1

Due to the fact that the MOR215 receptor is a murine G-protein coupled receptor (GPCR), it cannot be directly applied in Saccharomyces cerevisiae. Consequently, we engineered a Gα protein that is capable of responding to the MOR215 receptor within the yeast system. We made modifications to the five amino acids at the C-terminus of the protein to facilitate its expression in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/binding
Parameters
None