Device

Part:BBa_K5127008:Design

Designed by: Yinuo Li   Group: iGEM24_BNDS-China   (2024-10-01)


VFA0359-pVFA0359-gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1809
    Illegal XhoI site found at 1062
    Illegal XhoI site found at 1818
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 110
    Illegal SapI site found at 404
    Illegal SapI.rc site found at 1101


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E. coli.


Source

N/A


References

N/A