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Part:BBa_K5040004

Designed by: TING YU LIN   Group: iGEM24_CCU-Taiwan   (2024-09-22)


RCORI105-ZNF motifs-RCORI65

The version is composed of RCORI-65, ZNF motifs, and RCORI-105.

RCORI-105 and RCORI-65 was obtained from iGEM 2023 CCU-Taiwan serve as the start point and stop point of rolling circle replication, respectively.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 106
    Illegal NheI site found at 244
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 106
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 106
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 293
  • 1000
    COMPATIBLE WITH RFC[1000]



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