Part:BBa_K5038043

PT7-LIRA_input210-T7TE

Usage and Biology

LIRA_input210 is the gene sequence that was used to generate hsa-miR-210-3p. hsa-miR-210-3p can be used in response to the detection of LIRA_H210 and initiate the expression of downstream genes. The principle is the same as LIRA_input01.

This device is used to express LIRA_input210 in E.coli to test the function of LIRA_H210_2/20, LIRA_H210_7/15 and LIRA_H210_10/12

Characterization

T7 promoter could be induced by IPTG, so the intracellularly concentration of LIRA_input210 produces a large difference between the two conditions of IPTG presence/absence. This composite part is equipped by the following plasmids and co-transformed with the corresponding H210 plasmid.

(Table 1. LIRA_input210 plasmids and the corresponding H210 plasmids which have different stem/loop ratios)

No.	LIRA_input210 plasmids	corresponding H210 plasmid
1	pCDFDuet-LIRA_input210	pCOLADuet-H210_2/20-linker-EGFP
2	pCDFDuet-LIRA_input210	pCOLADuet-H210_7/15-linker-EGFP
3	pCDFDuet-LIRA_input210	pCOLADuet-H210_10/12-linker-EGFP
4	pET-15b-LIRA_input210	pCOLADuet-H210_2/20-linker-EGFP
5	pET-15b-LIRA_input210	pCOLADuet-H210_7/15-linker-EGFP
6	pET-15b-LIRA_input210	pCOLADuet-H210_10/12-linker-EGFP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]