Part:BBa_K5038011

LIRA_H210_2/20

Usage and Biology

LIRA_H210_2/20 is a LIRA system which could specifically detect hsa-miR-210-3p, and has the same detection principle to LIRA_H01. There is a complementary sequence of hsa-miR-210-3p on the loop of the hairpin structure, and the RBS and initiation codon AUG will be exposed when the complementary sequence is bound to the target RNA, thereby activates translation.

"2/20" indicates that this LIRA system has a stem-loop ratio of 2/20 when bound to the corresponding input, i.e., in the input sequence, 2 bases are bound to the stem of the LIRA hairpin and 20 bases are bound to the loop.

(Figure 1. Schematic of LIRA_H210_2/20)

Blue: miRNA binding site, miR-210-3p binds to this area
Purple: RBS
Yellow: AUG, initiation codon

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 26
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 14