RNA
Part:BBa_K5038008
Designed by: Qiyu Tan Group: iGEM24_CJUH-JLU-China (2024-09-14)
LIRA_H142_3/20
Usage and Biology
LIRA_H142_3/20 is a LIRA system which could specifically detect hsa-miR-142-3p, and has the same detection principle to LIRA_H01. There is a complementary sequence of hsa-miR-142-3p on the loop of the hairpin structure, and the RBS and initiation codon AUG will be exposed when the complementary sequence is bound to the target RNA, thereby activates translation.
"3/20" indicates that this LIRA system has a stem-loop ratio of 3/20 when bound to the corresponding input, i.e., in the input sequence, 3 bases are bound to the stem of the LIRA hairpin and 20 bases are bound to the loop.
(Figure 1. Schematic of LIRA_H142_3/20)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |