Primer

Part:BBa_K5007016:Design

Designed by: Pedro Ribeiro Faria   Group: iGEM23_USP-EEL-Brazil   (2023-10-06)


pRS425s R-primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer designed for cloning part BBa_K5007008 into pRS425s plasmid through Gibson Assembly, inside the LacZɑ gene cloning region. This primer linearizes the plasmid, generating the homology regions needed for the assembly method.


Source

This primer was designed internally and synthesized for use in PCR reactions.


References