Part:BBa_K4907034

bcsB

Biology

Bacterial cellulose (BC) considered as an important biopolymer because of its many more particular properties compared to plant cellulose. In cellulose-producing bacterial strains, most prominent of which is Gluconoacetobacter hansenii, BC biosynthesis is orchestrated by four genes; namely A, B, C, and D comprising a bacterial cellulose synthase (bcs) operon, preceded by two "regulatory" fragments. In some studies, it noted that BcsA and BcsB are two catalytic subunits that are present in all Bcs enzymes. Only bcsAB fragments are enough for effective in vitro BC production.When bcsA is activated by c-di-GMP, it incorporates glucose units into a cellulose chain in the cytoplasm using UDP glucose as a substrate. BcsB guides the glucan chain through the periplasm (1,2).

Reference

1. E. Sajadi et al., Increased cellulose production by heterologous expression of bcsA and B genes from Gluconacetobacterxylinus in E. coli Nissle 1917. Bioprocess. Biosyst. Eng. 42, 2023-2034 (2019).
2. S. S. Al-Janabi et al., Stable, efficient, and cost-effective system for the biosynthesis of recombinant bacterial cellulose in Escherichia coli DH5α platform. J. Genet. Eng. Biotechnol. 20, 90 (2022).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 687
  Illegal NheI site found at 802
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1690
  Illegal BamHI site found at 165
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 909
  Illegal NgoMIV site found at 1441
  Illegal AgeI site found at 40
  Illegal AgeI site found at 1000
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1179