Part:BBa_K4839015
WPRE
The Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) is a molecular tool widely utilized in molecular biology and biotechnology to enhance the expression of exogenous genes in eukaryotic cells. Derived from the genome of the Woodchuck Hepatitis Virus, this regulatory element has become an essential component in genetic engineering and gene expression studies.
Origins and Structure
The WPRE element was first discovered in the context of the Woodchuck Hepatitis Virus (WHV), a virus belonging to the Hepadnaviridae family. It is a compact regulatory element with a unique structure, consisting of approximately 325 nucleotides. Its origin in a viral genome has made it an attractive candidate for improving the stability and expression of transgenes in eukaryotic cells.
Function of WPRE
The primary function of the WPRE element is to enhance the stability and expression of exogenous genes in eukaryotic cells. It exerts its influence primarily at the post-transcriptional level, playing a pivotal role in the regulation of RNA stability, nuclear export, and overall gene expression. Here are some key mechanisms through which WPRE accomplishes its functions:
RNA Stability Enhancement: WPRE is known to increase the stability of the mRNA transcribed from the gene of interest. This is achieved through mechanisms that protect the mRNA molecule from degradation within the cytoplasm.
Nuclear Export Facilitation: WPRE assists in the efficient transport of mRNA from the nucleus to the cytoplasm. This process is crucial for the subsequent steps of translation and protein synthesis.
Enhanced Protein Expression: By improving mRNA stability and facilitating nuclear export, WPRE contributes to higher levels of protein expression from the target gene. This has significant implications for applications where robust protein production is required.
Applications of WPRE
The versatility of WPRE makes it an invaluable tool in various molecular biology and biotechnology applications. Some of the key applications include:
Gene Delivery: WPRE is often incorporated into gene expression vectors used for gene therapy and gene delivery. Its presence ensures that the therapeutic or target gene is expressed at sufficient levels to achieve the desired biological effect.
Biotechnology: In biotechnology, WPRE is used to enhance the production of recombinant proteins, antibodies, and other biopharmaceuticals in cell culture systems. This can lead to increased yields and cost-effective production processes.
Gene Expression Studies: Researchers employ WPRE to study gene expression and regulation. By ensuring stable and high-level expression of reporter genes, it helps elucidate the mechanisms underlying gene control.
Viral Vectors: WPRE is integrated into viral vectors, such as lentiviral vectors, adenoviral vectors, and retroviral vectors, to improve their efficiency in delivering therapeutic genes and gene editing tools into target cells.
In conclusion, the Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) stands as a fundamental molecular tool in the field of molecular biology and biotechnology. Its ability to enhance the stability and expression of exogenous genes has far-reaching implications, from gene therapy to basic research. By leveraging the mechanisms embedded within this regulatory element, scientists have unlocked new possibilities for precise and efficient control of gene expression in eukaryotic cells. As our understanding of gene regulation deepens and biotechnology continues to advance, WPRE remains a key player in shaping the future of genetic engineering and gene-based therapies.
[1]https://signagen.com/blog/2015/10/21/what-is-wpre/
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
- 1000COMPATIBLE WITH RFC[1000]
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