Primer

Part:BBa_K4816001:Design

Designed by: Julia Alexander   Group: iGEM23_Bonn-Rheinbach   (2023-09-25)


napA primer forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Specific binding to napA gene. Containing of restriction site for restriction digest and cloning into plasmid vector after PCR amplification.


Source

Sequence was designed with the DNA sequence for the napA gene of Paracoccus denitrificans found on KEGG https://www.genome.jp/entry/pde:Pden_4721

References