Part:BBa_K4788001

FadR transcription factor

codes FadR, it is a transcription factor that can bind to the FadBA promoter, inhibit initiation, and be released by oleoyl CoA metabolized from oleic acid. For specific information on oleic acid inducers, please refer to BBa_K4788002

Usage and Biology

It is a basic coding protein component that can combine with the FadO operator and FadBA promoter gene to form an oleic acid inducer to perform rich functions.

After forming composite components

The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects.

Reference

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]