Coding

Part:BBa_K4769207:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


eps operon coding sequence from B. subtilis 3610


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2173
    Illegal BglII site found at 1580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1357
    Illegal SapI.rc site found at 1525


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: atgaatgagaatatgagtttcaaagaattatatgc (+ desired overhangs) RV: gtctactttttctgtaatgctgtctg (+ desired overhangs)


References